Methods to determine the proportion of Cytosine methylation (5mC), the most common type of base modification in DNA.
NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
epigenetics illumina CpG WGBS EM-seq library preparation methylationSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
library preparation methylation epigenetics illumina CpG WGBS Bisulphite single-stranded DNA ssDNANanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
hifi de novo iso seq sv revio smrt assembly pacbio methylation ampliconThe EPIC BeadChip array allows for the interrogation over 935,000 methylation sites quantitatively across the genome at single-nucleotide resolution.
methylation epigenetics illumina dna array infinium CpGRuns with methylation sequencing data. It pre-processes raw data from FastQ inputs, aligns the reads and performs extensive quality-control on the results, using either Bismark or bwa-meth/MethylDackel.
methylseq Methyl-Seq BS Bisulfite