Full-length sequencing of RNA transcripts can be used to investigate splicing isoforms and fusion-gene transcripts, as well as annotation of de novo genomes.
Combining short & long reads at the single cell level.
library preparation single cell long-read illumina ont chromium 10x Genomics full transcriptNanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.
long-read nanopore assemblyNanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
de novo iso seq sv revio smrt assembly pacbio methylation amplicon hifiWith long and accurate HiFi reads, you can characterize the full diversity of the transcriptome
pacbio iso-seq hifi kinnex