NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
epigenetics illumina CpG WGBS EM-seq library preparation methylationProduction of high-quality proximity ligation libraries, using two restriction enzymes.
TADs illumina de novo chromatin scaffolding library preparation epigeneticsA method to identify open chromatin regions, such as promotor areas, using a transposase.
ATACseq ATAC-seq Omni-ATAC TN5 ATAC open chromatin epigenetics illuminaProfiling of chromatin accessibility at the single cell level.
epigenetics single cell illumina ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparationA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
scaffolding library preparation epigenetics TADs illumina de novo chromatinSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
Bisulphite single-stranded DNA ssDNA library preparation methylation epigenetics illumina CpG WGBSmiRNA libraries from very low input total RNA samples & degraded total RNA.
library preparation epigenetics RNA illuminaThe EPIC BeadChip array allows for the interrogation over 935,000 methylation sites quantitatively across the genome at single-nucleotide resolution.
dna array infinium CpG methylation epigenetics illuminaOxford Nanopore sequencers perform single-molecule long read sequencing of DNA and RNA
nanopore long reads ont direct RNA epigenetics