The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeProfiling of gene expression levels at single-cell resolution.
transcriptomics single cell illumina rnaseq smartseq3 SS3 single-cell library preparationNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
library preparation methylation epigenetics illumina CpG WGBS EM-seqRNA sequencing of mRNAs selected through poly-A enrichment.
library preparation transcriptomics RNA truseq mRNA illumina RNA-SeqRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
library preparation RNA truseq illumina rnaseq RNA-SeqTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
library preparation transcriptomics RNA mRNA illumina RNA-SeqProduction of high-quality proximity ligation libraries, using two restriction enzymes.
chromatin scaffolding library preparation epigenetics TADs illumina de novoSingle-cell profiling of gene expression levels on a large number of cells.
mRNA illumina RNA-Seq scale library preparation transcriptomics single cell RNACombining short & long reads at the single cell level.
chromium 10x Genomics full transcript library preparation single cell long-read illumina ontProfiling of chromatin accessibility at the single cell level.
10x Genomics ATAC library preparation epigenetics single cell illumina ATAC-seq dna chromium chromatin