Associated Keywords: library preparation

HLA Typing with Nanopore Sequencing

The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.

Smart-seq3

Profiling of gene expression levels at single-cell resolution.

Enzymatic Methyl-seq (EM-seq)

NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.

Illumina TruSeq Stranded mRNA

RNA sequencing of mRNAs selected through poly-A enrichment.

Illumina TruSeq Stranded RNA without selection

RNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.

Illumina Stranded total RNA Prep (Ribo-Zero Plus)

Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.

Arima HiC

Production of high-quality proximity ligation libraries, using two restriction enzymes.

Scale Biosciences

Single-cell profiling of gene expression levels on a large number of cells.