The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeProfiling of gene expression levels at single-cell resolution.
library preparation transcriptomics single cell illumina rnaseq smartseq3 SS3 single-cellNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
WGBS EM-seq library preparation methylation epigenetics illumina CpGRNA sequencing of mRNAs selected through poly-A enrichment.
illumina RNA-Seq library preparation transcriptomics RNA truseq mRNARNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
rnaseq RNA-Seq library preparation RNA truseq illuminaTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
RNA-Seq library preparation transcriptomics RNA mRNA illuminaProduction of high-quality proximity ligation libraries, using two restriction enzymes.
illumina de novo chromatin scaffolding library preparation epigenetics TADsSingle-cell profiling of gene expression levels on a large number of cells.
transcriptomics single cell RNA mRNA illumina RNA-Seq scale library preparationCombining short & long reads at the single cell level.
long-read illumina ont chromium 10x Genomics full transcript library preparation single cellProfiling of chromatin accessibility at the single cell level.
ATAC-seq dna chromium chromatin 10x Genomics ATAC library preparation epigenetics single cell illumina