NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
methylation epigenetics illumina CpG WGBS EM-seq library preparationRNA sequencing of mRNAs selected through poly-A enrichment.
RNA truseq mRNA illumina RNA-Seq library preparation transcriptomicsRNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.
RNA truseq illumina rnaseq RNA-Seq library preparationTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
transcriptomics RNA mRNA illumina RNA-Seq library preparationCombining short & long reads at the single cell level.
chromium 10x Genomics full transcript library preparation single cell long-read illumina ontProfiling of chromatin accessibility at the single cell level.
10x Genomics ATAC library preparation epigenetics single cell illumina ATAC-seq dna chromium chromatinProfiling of 3´gene expression and chromatin accessibility in the same cell.
mRNA illumina ATAC-seq RNA-Seq ATAC chromium library preparation 10x Genomics transcriptomics single cell RNAProfiling of gene expression levels at single-cell resolution.
10x Genomics library preparation transcriptomics single cell RNA mRNA illumina RNA-Seq chromiumSingle-cell profiling of gene expression levels on fixed cells.
single cell RNA mRNA illumina RNA-Seq chromium 10x Genomics fixed cells library preparation transcriptomicsA proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.
library preparation epigenetics TADs illumina de novo chromatin scaffolding