DNA resequencing

High-throughput full-genome or targeted sequencing of DNA from organisms with an existing reference genome.

Whole genome resequencing
Sequence the entire genome, including non-coding and intergenic regions. Gives high resolution data for identification and study of genomic variation.
Targeted resequencing
Methods to identify and study variation within specific genomic regions of interest.
Whole exome sequencing
Targeted enrichment and sequencing of the protein-coding regions of the genome.
HLA Typing with Nanopore Sequencing

The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.

Illumina 16S sequencing

Sequencing of the 16S gene to study bacterial diversity and composition in a sample.

Illumina amplicon sequencing

Sequencing of PCR amplicons to study genetic variation within small target regions.

Illumina DNA

Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.

Illumina DNA PCR-Free

Method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

ThruPLEX DNA-seq

Library preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.

Nanopore DNA sequencing

Nanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

RAD-sequencing

Genotyping-by-sequencing without prior genome information.

Twist Bioscience Human Exome panel

A platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.

Nanopore analysis

Quality control, Basecalling and multiplexing of sequencing reads generated by Oxford Nanopore sequencers.

PromethION secondary analysis

Additional compute intensive nanopore raw data processing services provided by NGI

Illumina QC analysis

Basic quality-control monitoring of Illumina FastQ sequence data.

Whole Genome/Exome Sequencing analysis

Runs with illumina DNA-sequencing data, WGS or targeted sequencing e.g. WES. Aligns to the reference genome, gives QC metrics, does variant-calling and finishes with annotation.