High-throughput full-genome or targeted sequencing of DNA from organisms with an existing reference genome.
The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.
library preparation nanoporeSequencing of the 16S gene to study bacterial diversity and composition in a sample.
illumina 16S amplicon targeted library preparationSequencing of PCR amplicons to study genetic variation within small target regions.
library preparation illumina 16S amplicon targetedLow cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.
library preparation genome illumina WGS dna nextera normalizationMethod for shotgun DNA libraries used for whole genome sequencing and metagenomics.
genome illumina WGS dna tagmentation PCR-free library preparationGold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.
truseq genome illumina WGS dna library preparationLibrary preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.
truseq genome illumina WGS dna library preparationLibrary preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.
library preparation genome illumina WGS dnaNanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.
assembly long-read nanoporePacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.
methylation amplicon hifi de novo iso seq sv revio smrt assembly pacbioGenotyping-by-sequencing without prior genome information.
genome illumina wholegenome dna polymorphism library preparationA platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.
library preparation illumina dna exome targetedQuality control, Basecalling and multiplexing of sequencing reads generated by Oxford Nanopore sequencers.
long-read nanoporeAdditional compute intensive nanopore raw data processing services provided by NGI
basecalling pod5 methylation base modificationsBasic quality-control monitoring of Illumina FastQ sequence data.
fastq screen checkqc QC fastqcRuns with illumina DNA-sequencing data, WGS or targeted sequencing e.g. WES. Aligns to the reference genome, gives QC metrics, does variant-calling and finishes with annotation.
WGS sarek cancer human data WES