Status: NGI Uppsala

This protocol is available at NGI Uppsala.

Olink Explore HT multiplex protein analysis

Method for multiplex protein biomarker analysis (5400 proeins) with sequencing readout using the Illumina NovaSeq platform.

Olink Explore 384-3072 multiplex protein analysis

Method for multiplex protein biomarker analysis with sequencing readout using the Illumina NovaSeq platform.

Enzymatic Methyl-seq (EM-seq)

NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.

Illumina Stranded total RNA Prep (Ribo-Zero Plus)

Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.

HMW DNA extraction

NGI now offers High Molecular Weight (HMW) DNA extraction as a service for sequencing projects at or in collaboration with NGI and SciLifeLab.

SPlinted Ligation Adapter Tagging (SPLAT) for Whole Genome Bisulphite Sequencing (WGBS)

SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

Twist Bioscience Human Exome panel

A platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.