This protocol is available at NGI Uppsala.
Design targeted sequencing panels for your regions of interest. Twist Bioscience provides the probes, and NGI handles QC, library prep, and sequencing.
methylation RNA dna twist bioscienceMethod for multiplex protein biomarker analysis (>1000 proteins) with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink RevealMethod for multiplex protein biomarker analysis (5400 proteins) with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink HTMethod for multiplex protein biomarker analysis with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink ExploreNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
library preparation methylation epigenetics illumina CpG WGBS EM-seqTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
library preparation transcriptomics RNA mRNA illumina RNA-SeqNGI now offers High Molecular Weight (HMW) DNA extraction as a service for sequencing projects at or in collaboration with NGI and SciLifeLab.
dna extraction hmw hmw dnaG-SPLAT is a library preparation technique designed for low-quality or limited-input DNA, with applications in whole-genome sequencing, metagenomics, and and other challenging sequencing projects.
genome illumina WGS dna single-stranded DNA ssDNA library preparationSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
ssDNA library preparation methylation epigenetics illumina CpG WGBS Bisulphite single-stranded DNA