This protocol is available at NGI Uppsala.
Design targeted sequencing panels for your regions of interest. Twist Bioscience provides the probes, and NGI handles QC, library prep, and sequencing.
twist bioscience methylation RNA dnaMethod for multiplex protein biomarker analysis (>1000 proteins) with sequencing readout using the Illumina NovaSeq platform.
Olink biomarker Olink Reveal proteinMethod for multiplex protein biomarker analysis (5400 proteins) with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink HTMethod for multiplex protein biomarker analysis with sequencing readout using the Illumina NovaSeq platform.
protein Olink biomarker Olink ExploreNEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.
illumina CpG WGBS EM-seq library preparation methylation epigeneticsTotal RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.
illumina RNA-Seq library preparation transcriptomics RNA mRNANGI now offers High Molecular Weight (HMW) DNA extraction as a service for sequencing projects at or in collaboration with NGI and SciLifeLab.
extraction hmw hmw dna dnaG-SPLAT is a library preparation technique designed for low-quality or limited-input DNA, with applications in whole-genome sequencing, metagenomics, and and other challenging sequencing projects.
WGS dna single-stranded DNA ssDNA library preparation genome illuminaSPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA
epigenetics illumina CpG WGBS Bisulphite single-stranded DNA ssDNA library preparation methylation