Methods

Enzymatic Methyl-seq (EM-seq)

NEBNext EM-Seq kit is an alternative to whole-genome bisulfite sequencing (WGBS). The enzymatic conversion of unmethylated cytosines in EM-seq is more gentle to DNA than WGBS and results in libraries with more even genome coverage and better coverage of CpG sites across the genome.

HLA Typing with Nanopore Sequencing

The complexity and polymorphic nature of the 4 Mb region makes it challenging to resolve using short-read sequencing technology. Nanopore (ONT) sequencing, can provide long reads that overcome these challenges, enabling the identification of variants that could represent new biomarkers, unambiguous haplotype phasing of single-nucleotide variants (SNVs), and the potential for increased accuracy of HLA typing at a higher resolution.

Olink Explore 384-3072 multiplex protein analysis

Method for multiplex protein biomarker analysis with sequencing readout using the Illumina NovaSeq platform.

Olink Explore HT multiplex protein analysis

Method for multiplex protein biomarker analysis (5400 proteins) with sequencing readout using the Illumina NovaSeq platform.

Olink Reveal multiplex protein analysis

Method for multiplex protein biomarker analysis (>1000 proteins) with sequencing readout using the Illumina NovaSeq platform.

Illumina TruSeq Stranded mRNA

RNA sequencing of mRNAs selected through poly-A enrichment.

Illumina TruSeq Stranded RNA without selection

RNA sequencing of either all RNAs in a sample, or of a RNA sample depleted of for example rRNA by the user.

Illumina Stranded total RNA Prep (Ribo-Zero Plus)

Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples.

Visium CytAssist for FFPE samples (probe based)

We now offer spatially resolved transcriptomics through the 10X Genomics Visium CytAssist, which combines histology with probe-based transcriptomics in a spatial context.

Visium CytAssist for Fresh Frozen and Fixed Frozen samples (probe based)

We now offer spatially resolved transcriptomics through the 10X Genomics Visium CytAssist, which combines histology with probe-based transcriptomics in a spatial context.

Visium for Fresh Frozen samples (poly-A based)

We offer spatial transcriptomics through the 10X Genomics Visium method. The method combines histology with unbiased transcriptomics in a spatial context.

Visium HD CytAssist for FF and FxF samples (probe based)

We now offer high resolution spatially resolved transcriptomics through the 10X Genomics Visium HD CytAssist, which combines histology with probe-based transcriptomics in HD spatial context

Visium HD CytAssist for FFPE samples (probe based)

We now offer high resolution spatially resolved transcriptomics through the 10X Genomics Visium HD CytAssist, which combines histology with probe-based transcriptomics in HD spatial context

Omni-ATAC

A method to identify open chromatin regions, such as promotor areas, using a transposase.

Scale Biosciences

Single-cell profiling of gene expression levels on a large number of cells.

Dovetail Omni-C

A proximity-ligation protocol using a sequence-independent endonuclease, generating data for TAD identification and scaffolding.

HMW DNA extraction

NGI now offers High Molecular Weight (HMW) DNA extraction as a service for sequencing projects at or in collaboration with NGI and SciLifeLab.

Illumina 16S sequencing

Sequencing of the 16S gene to study bacterial diversity and composition in a sample.

Illumina amplicon sequencing

Sequencing of PCR amplicons to study genetic variation within small target regions.

SPlinted Ligation Adapter Tagging (SPLAT) for Whole Genome Bisulphite Sequencing (WGBS)

SPLAT is an in-house developed WGBS library preparation method. This approach enables quick and efficient preparation of WGBS libraries from low-input DNA

Illumina DNA

Low cost library preparation option for gDNA based on bead-linked transposase. Only for full plates of samples.

Illumina sequencing

NGI can sequence user-prepared libraries on all Illumina instruments. User-prepared libraries orders can only be whole-lane orders. Libraries with custom setups and or custom primers can only be whole flow cells.

Illumina DNA PCR-Free

Method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA PCR-free

Gold standard method for shotgun DNA libraries used for whole genome sequencing and metagenomics.

Illumina TruSeq DNA Nano

Library preparation from limited input DNA, used in whole genome sequencing and metagenomics etc.

ThruPLEX DNA-seq

Library preparation for DNA, ideal for preparing libraries from small amounts of input material. Works well for shotgun libraries, ChIP DNA and FFPE samples, amongst others.

Nanopore cDNA sequencing

Nanopore cDNA sequencing is able to sequence entire transcripts in one go, ideal for detecting isoforms and fusions events.

Nanopore DNA sequencing

Nanopore instruments can sequence very long continuous fragments of DNA. Sequencing native DNA allows detection of base modifications.

Nanopore Direct RNA sequencing

Nanopore direct RNA sequencing is able to sequence entire transcripts from native RNA, opening up opportunities to detect RNA modifications.

PacBio SMRT sequencing

PacBio SMRT sequencing generates reads tens of kilobases in length enabling high quality genome assembly, structural variant analysis, amplicon resequencing, full-length transcript isoform sequencing, full-length 16S rRNA sequencing and amplification free epigenetic characterization.

QIAseq miRNA

miRNA libraries from very low input total RNA samples & degraded total RNA.

RAD-sequencing

Genotyping-by-sequencing without prior genome information.

TaKaRa SMARTer pico RNA kit

The Takara SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian kit is specifically designed for very low input total RNA samples. It also works with degraded total RNA.

Twist Bioscience Human Exome panel

A platform for human whole exome sequencing (WES) using target enrichment and library preparation for next generation sequencing.

Illumina Infinium SNP Genotyping Arrays

We offer a broad range of microarray options for high-throughput genotyping solutions based on SNP complexity and organism.

Illumina Infinium DNA Methylation EPIC Array

The EPIC BeadChip array allows for the interrogation over 935,000 methylation sites quantitatively across the genome at single-nucleotide resolution.